The technique used to amplify DNA in a test tube is: MCQ with Answer and Explanation

The technique used to amplify DNA in a test tube is:
A. Southern blotting
B. Sequencing
C. PCR
D. Gel electrophoresis
Answer: Option C
Solution (By JKSSB Mock Tests)
Polymerase chain reaction (PCR) uses Taq polymerase, primers, and thermal cycling to make millions of copies of a specific DNA sequence. Southern blotting detects DNA, electrophoresis separates, sequencing reads bases.

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Practice More Biology Questions

Question #1
The disorder characterized by repetitive seizures due to abnormal brain electrical activity is:
A. Stroke
B. Epilepsy
C. Multiple sclerosis
D. Meningitis

Correct Answer: Option B


Explanation:
Epilepsy is a neurological disorder marked by recurrent seizures caused by excessive and synchronous neuronal firing. Stroke is from interrupted blood supply, MS is demyelination, meningitis is inflammation of meninges.

This question belongs to: Science Biology
Question #2
The type of fossil formed when minerals replace organic material, preserving the original structure, is called:
A. Mold fossil
B. Cast fossil
C. Trace fossil
D. Petrified fossil

Correct Answer: Option D


Explanation:
Petrified fossils (permineralization) form when dissolved minerals infiltrate the porous tissues of dead organisms and harden, turning the remains into stone while preserving internal structure. Mold and cast fossils are impressions, trace fossils are tracks or burrows. Petrified wood is a common example.

This question belongs to: Science Biology
Question #3
The term 'genetically modified organism' (GMO) refers to:
A. An organism produced by selective breeding
B. An organism whose DNA has been altered using genetic engineering
C. A hybrid organism produced by cross-pollination
D. A cloned organism

Correct Answer: Option B


Explanation:
GMOs are organisms whose genetic material has been modified through recombinant DNA technology, introducing or deleting specific genes. Selective breeding and cross-pollination are traditional methods that do not directly manipulate DNA. Cloning produces genetically identical copies without necessarily altering the genome. Examples: Bt cotton, Golden rice.

This question belongs to: Science Biology