The technique used to amplify DNA in a test tube is: MCQ with Answer and Explanation

The technique used to amplify DNA in a test tube is:
A. PCR
B. Sequencing
C. Gel electrophoresis
D. Southern blotting
Answer: Option A
Solution (By JKSSB Mock Tests)
Polymerase chain reaction (PCR) uses Taq polymerase, primers, and thermal cycling to make millions of copies of a specific DNA sequence. Southern blotting detects DNA, electrophoresis separates, sequencing reads bases.

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Practice More Biology Questions

Question #1
The theory of chemical evolution of life was proposed by:
A. Oparin and Haldane
B. Charles Darwin
C. Louis Pasteur
D. Stanley Miller

Correct Answer: Option A


Explanation:
The chemical evolution theory, also known as the biochemical theory of the origin of life, was proposed independently by Alexander Oparin (1924) and J.B.S. Haldane (1929). They proposed that life originated from pre-existing non-living organic molecules (like RNA, proteins, lipids) through a series of complex chemical reactions in the primitive reducing atmosphere of Earth.

This question belongs to: Science Biology
Question #2
Consider statements about scientists: 1. Carolus Linnaeus developed binomial nomenclature. 2. Louis Pasteur developed the rabies vaccine. 3. Gregor Mendel discovered the structure of DNA. Which are correct?
A. 2 and 3 only
B. 1 and 2 only
C. 1, 2 and 3
D. 1 and 3 only

Correct Answer: Option B


Explanation:
Carolus Linnaeus, a Swedish botanist, formalized the system of binomial nomenclature for naming organisms. Louis Pasteur made immense contributions to microbiology, including the development of the first effective vaccine against rabies. Gregor Mendel is the father of genetics, known for his laws of inheritance; the structure of DNA was discovered by Watson and Crick. Thus, statements 1 and 2 are correct.

This question belongs to: Science Biology
Question #3
Assertion (A): The cell wall of plants is freely permeable.
Reason (R): It allows water and dissolved substances to pass through.
A. Both A and R are true, but R is not the correct explanation.
B. A false, R true.
C. Both A and R are true, and R is the correct explanation.
D. A true, R false.

Correct Answer: Option C


Explanation:
The cell wall is porous and does not regulate entry; the plasma membrane is selectively permeable. Thus, the wall is freely permeable, and the reason explains it.

This question belongs to: Science Biology