In biotechnology, the 'PCR' (Polymerase Chain Reaction) technique requires a thermostable DNA polymerase. The most commonly used enzyme for this is: MCQ with Answer and Explanation
In biotechnology, the 'PCR' (Polymerase Chain Reaction) technique requires a thermostable DNA polymerase. The most commonly used enzyme for this is:
A. Reverse transcriptase
B. Taq polymerase
C. T7 polymerase
D. DNA polymerase I
Answer: Option B
Solution (By JKSSB Mock Tests)
PCR involves repeated cycles of high-temperature denaturation (around 94°C) to separate the DNA strands. Normal DNA polymerases would be destroyed at these temperatures. Therefore, a thermostable DNA polymerase is required. Taq polymerase, isolated from the thermophilic bacterium Thermus aquaticus found in hot springs, is the most commonly used enzyme because it can withstand the high denaturation temperatures without losing its activity, eliminating the need to add fresh enzyme after each cycle.
Explanation:
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Explanation:
The pancreas, liver, and salivary glands are accessory digestive glands that secrete enzymes or substances aiding digestion into the alimentary canal. The pancreas produces pancreatic juice (enzymes and bicarbonate) released into the duodenum. The stomach and small intestine are part of the main digestive tract, and the esophagus is a conduit for food.
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