PCR is a technique used to: MCQ with Answer and Explanation

PCR is a technique used to:
A. Sequence DNA
B. Separate DNA fragments
C. Cut DNA
D. Amplify a specific DNA sequence
Answer: Option D
Solution (By JKSSB Mock Tests)
Polymerase chain reaction (PCR) uses Taq polymerase and primers to exponentially amplify a target DNA segment in vitro.

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Practice More Biology Questions

Question #1
Which of the following microorganisms is used for the production of citric acid in the industry?
A. Saccharomyces cerevisiae
B. Aspergillus niger
C. Rhizobium leguminosarum
D. Penicillium chrysogenum

Correct Answer: Option B


Explanation:
Aspergillus niger is a fungus widely used for the industrial production of citric acid (a common food additive) through fermentation. Penicillium chrysogenum produces penicillin, Saccharomyces cerevisiae is used in baking and brewing, Rhizobium is a nitrogen-fixing bacterium. Citric acid is used as a preservative and flavor enhancer.

This question belongs to: Science Biology
Question #2
The 'Bohr effect' and the 'Haldane effect' are both phenomena related to the transport of gases in the blood. The Bohr effect facilitates the loading of oxygen in the:
A. Lungs
B. Liver
C. Tissues
D. Kidneys

Correct Answer: Option A


Explanation:
The Bohr effect describes how an increase in pCO2 or a decrease in pH (increase in H+) reduces hemoglobin's affinity for oxygen, shifting the oxygen dissociation curve to the right and promoting oxygen unloading. Conversely, in the lungs, where pCO2 is low and pH is relatively higher (more alkaline), the Bohr effect works in reverse: hemoglobin's affinity for oxygen increases, facilitating the loading (binding) of oxygen to hemoglobin. The Haldane effect describes the reciprocal relationship for CO2 transport.

This question belongs to: Science Biology
Question #3
The 'powerhouse of the cell' is the:
A. Ribosome
B. Mitochondria
C. Nucleus
D. Lysosome

Correct Answer: Option B


Explanation:
Mitochondria generate most of the cell's ATP through oxidative phosphorylation. They have a double membrane and their own DNA.

This question belongs to: Science Biology