The enzyme used to join DNA fragments in genetic engineering is: MCQ with Answer and Explanation

The enzyme used to join DNA fragments in genetic engineering is:
A. DNA ligase
B. Helicase
C. Restriction enzyme
D. DNA polymerase
Answer: Option A
Solution (By JKSSB Mock Tests)
DNA ligase seals the phosphodiester bonds between adjacent DNA fragments, joining them together. In recombinant DNA technology, it is used to ligate the gene of interest into a vector. Restriction enzymes cut DNA, DNA polymerase synthesizes DNA, and helicase unwinds the double helix. Ligase is essential for finalizing the recombinant DNA molecule.

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Practice More Biology Questions

Question #1
The type of epithelial tissue that lines the respiratory tract and moves mucus is:
A. Squamous epithelium
B. Cuboidal epithelium
C. Ciliated columnar epithelium
D. Transitional epithelium

Correct Answer: Option C


Explanation:
Ciliated pseudostratified columnar epithelium (with goblet cells) lines the trachea and bronchi; the cilia beat in coordinated waves to move mucus and trapped particles out of the airways. Squamous epithelium is flat (alveoli), cuboidal in kidney tubules, transitional in urinary bladder.

This question belongs to: Science Biology
Question #2
Consider statements about human diseases: 1. Bacterial diseases can be treated with antibiotics. 2. Viral diseases are often prevented by vaccines. 3. Fungal diseases are caused by protozoa. Which are correct?
A. 1 and 2 only
B. 2 and 3 only
C. 1, 2 and 3
D. 1 and 3 only

Correct Answer: Option A


Explanation:
Bacterial infections (like tuberculosis, pneumonia) are effectively treated with antibiotics, which target specific bacterial structures or metabolic pathways. Many viral diseases (like polio, measles, influenza) can be prevented through vaccination, which stimulates the immune system to develop memory against the virus. Fungal diseases (like ringworm, candidiasis) are caused by fungi, not protozoa; protozoa cause diseases like malaria and amoebiasis. Thus, statements 1 and 2 are correct.

This question belongs to: Science Biology
Question #3
Assertion (A): Bacteria can be killed by antibiotics like penicillin.
Reason (R): Penicillin inhibits the synthesis of bacterial cell wall.
A. A true, R false.
B. Both A and R are true, and R is the correct explanation.
C. A false, R true.
D. Both A and R are true, but R is not the correct explanation.

Correct Answer: Option B


Explanation:
Penicillin targets the transpeptidase enzyme, blocking peptidoglycan cross-linking, leading to cell lysis. This is the mechanism, so R correctly explains A.

This question belongs to: Science Biology