The process of separating proteins based on their net charge is: MCQ with Answer and Explanation

The process of separating proteins based on their net charge is:
A. Gel filtration chromatography
B. Ion exchange chromatography
C. Affinity chromatography
D. SDS-PAGE
Answer: Option B
Solution (By JKSSB Mock Tests)
Ion exchange chromatography separates proteins based on their net surface charge; positively charged proteins bind to cation exchangers, negatively to anion exchangers. Gel filtration separates by size, affinity by specific binding, SDS-PAGE by molecular weight.

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Practice More Biology Questions

Question #1
The biodiversity hotspot concept was introduced by:
A. Norman Myers
B. E.O. Wilson
C. Charles Darwin
D. Alexander von Humboldt

Correct Answer: Option A


Explanation:
Norman Myers introduced the concept of biodiversity hotspots in 1988—regions with high endemic species and significant habitat loss (≥70% original vegetation lost). Examples: Western Ghats, Indo-Burma. Conservation efforts prioritize these areas. Darwin contributed evolution, Humboldt to biogeography, E.O. Wilson is known for sociobiology and biodiversity studies. Hotspots cover only 2.4% of Earth's land but hold >50% of plant species.

This question belongs to: Science Biology
Question #2
Consider statements about plant reproduction: 1. Pollination is the transfer of pollen to the stigma. 2. Double fertilization is unique to gymnosperms. 3. Seeds develop from ovules. Which are correct?
A. 2 and 3 only
B. 1, 2 and 3
C. 1 and 3 only
D. 1 and 2 only

Correct Answer: Option C


Explanation:
Pollination is the mechanism of transferring pollen grains from the anther to the stigma of a flower. After fertilization, the ovule develops into a seed and the ovary into a fruit. Double fertilization, involving syngamy and triple fusion, is a unique characteristic of angiosperms, not gymnosperms. Therefore, statements 1 and 3 are correct.

This question belongs to: Science Biology
Question #3
Vaccination provides which type of immunity?
A. Artificial active immunity
B. Natural active immunity
C. Natural passive immunity
D. Artificial passive immunity

Correct Answer: Option A


Explanation:
Vaccination involves the introduction of a weakened, killed, or partial pathogen (antigen) into the body. This stimulates the immune system to produce its own antibodies and memory cells without causing the disease. Since the body actively produces the immune response due to an artificial stimulus, it is called artificial active immunity.

This question belongs to: Science Biology