The process of 'Southern blotting' is a technique used in molecular biology to detect:
A. Specific RNA sequences in a sample
B. Specific DNA sequences in a sample
C. Lipids in a cell membrane
D. Specific proteins in a sample
Answer: Option B
Solution (By JKSSB Mock Tests)
Southern blotting, named after its inventor Edwin Southern, is a laboratory method used to detect specific DNA sequences in a complex DNA sample. The process involves digesting DNA with restriction enzymes, separating the fragments by gel electrophoresis, transferring (blotting) them onto a nylon or nitrocellulose membrane, and then hybridizing the membrane with a labeled, complementary DNA or RNA probe. Northern blotting detects RNA, and Western blotting detects proteins.
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