The process of 'Southern blotting' is a technique used in molecular biology to detect: MCQ with Answer and Explanation

The process of 'Southern blotting' is a technique used in molecular biology to detect:
A. Specific RNA sequences in a sample
B. Specific DNA sequences in a sample
C. Lipids in a cell membrane
D. Specific proteins in a sample
Answer: Option B
Solution (By JKSSB Mock Tests)
Southern blotting, named after its inventor Edwin Southern, is a laboratory method used to detect specific DNA sequences in a complex DNA sample. The process involves digesting DNA with restriction enzymes, separating the fragments by gel electrophoresis, transferring (blotting) them onto a nylon or nitrocellulose membrane, and then hybridizing the membrane with a labeled, complementary DNA or RNA probe. Northern blotting detects RNA, and Western blotting detects proteins.

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Practice More Biology Questions

Question #1
The process of cell division that produces four haploid daughter cells is:
A. Mitosis
B. Amitosis
C. Meiosis
D. Binary fission

Correct Answer: Option C


Explanation:
Meiosis consists of two successive divisions resulting in four genetically diverse haploid cells (gametes). Mitosis produces two identical diploid cells, amitosis is direct nuclear division, binary fission is prokaryotic asexual reproduction.

This question belongs to: Science Biology
Question #2
Which vitamin is essential for the absorption of calcium from the intestine?
A. Vitamin D
B. Vitamin B₁
C. Vitamin A
D. Vitamin C

Correct Answer: Option A


Explanation:
Vitamin D (calciferol) promotes the absorption of calcium and phosphorus from the small intestine by inducing synthesis of calcium-binding proteins. It is synthesized in the skin upon exposure to UV light and then activated in the liver and kidneys. Deficiency leads to rickets in children and osteomalacia in adults. Vitamin A is for vision, C for collagen, B₁ for energy metabolism.

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Question #3
The plant hormone that promotes the formation of abscission layers and fruit ripening is:
A. Gibberellin
B. Auxin
C. Ethylene
D. Cytokinin

Correct Answer: Option C


Explanation:
Ethylene is a gaseous hormone that accelerates ripening, promotes leaf and fruit abscission, and senescence. Auxin and cytokinin promote growth, gibberellin elongation. Ethylene is used commercially for fruit ripening.

This question belongs to: Science Biology