The technique of using radioactive probes to detect specific DNA sequences on a membrane is: MCQ with Answer and Explanation

The technique of using radioactive probes to detect specific DNA sequences on a membrane is:
A. ELISA
B. Flow cytometry
C. PCR
D. Southern blotting
Answer: Option D
Solution (By JKSSB Mock Tests)
Southern blotting involves separation of DNA fragments by gel electrophoresis, transfer to a membrane, and hybridization with a labeled probe. PCR amplifies DNA, ELISA detects proteins, flow cytometry analyzes cells.

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Practice More Biology Questions

Question #1
Which organelle is primarily responsible for the synthesis of lipids?
A. Smooth endoplasmic reticulum
B. Golgi apparatus
C. Rough endoplasmic reticulum
D. Lysosome

Correct Answer: Option A


Explanation:
Smooth endoplasmic reticulum (SER) lacks ribosomes and is the site of lipid synthesis, including phospholipids and steroids. It also detoxifies drugs and poisons. Rough ER synthesizes proteins, Golgi modifies and packages molecules, lysosomes degrade waste. SER is abundant in cells producing steroid hormones.

This question belongs to: Science Biology
Question #2
Which mineral is required for the formation of hemoglobin?
A. Iron
B. Calcium
C. Iodine
D. Zinc

Correct Answer: Option A


Explanation:
Iron (Fe²⁺) is the central atom of heme in hemoglobin and myoglobin, essential for oxygen transport. Deficiency leads to microcytic hypochromic anemia. Good sources: liver, meat, leafy greens.

This question belongs to: Science Biology
Question #3
Consider statements about human physiology: 1. The kidney filters blood to form urine. 2. The liver produces bile for fat digestion. 3. The spleen produces digestive enzymes. Which are correct?
A. 1 and 2 only
B. 1, 2 and 3
C. 2 and 3 only
D. 1 and 3 only

Correct Answer: Option A


Explanation:
The kidneys are the primary excretory organs, filtering blood through nephrons to remove waste products and excess water, forming urine. The liver is a multifunctional organ that produces bile, which is stored in the gall bladder and released into the small intestine to emulsify fats, aiding in their digestion. The spleen is a lymphoid organ involved in filtering blood and immune responses; it does not produce digestive enzymes (the pancreas does). Thus, statements 1 and 2 are correct.

This question belongs to: Science Biology