The technique that uses antibodies to detect specific proteins in a sample is: MCQ with Answer and Explanation

The technique that uses antibodies to detect specific proteins in a sample is:
A. Southern blotting
B. PCR
C. ELISA
D. Northern blotting
Answer: Option C
Solution (By JKSSB Mock Tests)
ELISA (enzyme-linked immunosorbent assay) uses enzyme-linked antibodies to detect and quantify specific antigens or antibodies in a sample. PCR amplifies DNA, Northern blotting detects RNA, Southern blotting DNA.

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Practice More Biology Questions

Question #1
Consider statements about human immunity: 1. Active immunity involves the body producing its own antibodies. 2. Passive immunity involves receiving pre-formed antibodies. 3. Vaccination provides passive immunity. Which are correct?
A. 1 and 2 only
B. 2 and 3 only
C. 1, 2 and 3
D. 1 and 3 only

Correct Answer: Option A


Explanation:
Active immunity occurs when the body's own immune system is stimulated by an antigen (either through infection or vaccination) to produce its own antibodies and memory cells, providing long-lasting protection. Passive immunity occurs when an individual is directly given pre-formed antibodies (like through mother's milk or antiserum), providing immediate but temporary protection. Vaccination stimulates the body to produce its own immune response, thus providing artificial active immunity, not passive. Thus, statements 1 and 2 are correct.

This question belongs to: Science Biology
Question #2
The scientist who developed the first effective polio vaccine was:
A. Louis Pasteur
B. Albert Sabin
C. Edward Jenner
D. Jonas Salk

Correct Answer: Option D


Explanation:
Jonas Salk developed the inactivated polio vaccine (IPV) in 1955, which was the first effective vaccine. Albert Sabin later developed the oral polio vaccine (OPV). Pasteur rabies, Jenner smallpox.

This question belongs to: Science Biology
Question #3
The 'Hormone Progesterone' is primarily responsible for maintaining pregnancy. During the menstrual cycle, its levels are highest during the:
A. Follicular (proliferative) phase
B. Menstrual phase
C. Luteal (secretory) phase
D. Ovulation phase

Correct Answer: Option C


Explanation:
Progesterone is the primary hormone of the luteal phase (also called the secretory phase) of the menstrual cycle. After ovulation, the ruptured Graafian follicle transforms into the corpus luteum, which secretes large amounts of progesterone. Progesterone prepares and maintains the uterine endometrium (thickening it, increasing blood supply, and stimulating glandular secretion) for the potential implantation of a fertilized egg. If pregnancy does not occur, the corpus luteum degenerates, progesterone levels drop, and menstruation begins.

This question belongs to: Science Biology