The technique that uses antibodies to detect specific proteins in a sample is:
A. Southern blotting
B. ELISA
C. Northern blotting
D. PCR
Answer: Option B
Solution (By JKSSB Mock Tests)
ELISA (enzyme-linked immunosorbent assay) uses enzyme-linked antibodies to detect and quantify specific antigens or antibodies in a sample. PCR amplifies DNA, Northern blotting detects RNA, Southern blotting DNA.
Explanation:
Pinocytosis ('cell drinking') is a form of endocytosis where the cell membrane invaginates to take in a small droplet of extracellular fluid and dissolved solutes. Phagocytosis is 'cell eating' of large particles, exocytosis is secretion, osmosis is water movement across a semipermeable membrane.
Explanation:
Semi-conservative replication means that when a new DNA double helix is formed, it consists of one original (parental) strand and one newly synthesized strand. This was proven by the Meselson-Stahl experiment in 1958 using isotopes of nitrogen (15N and 14N) in E. coli. This mechanism ensures genetic continuity.
Explanation:
'Survival of the fittest' was a phrase used by Herbert Spencer but adopted by Darwin to describe natural selection—organisms best adapted to the environment survive and reproduce. Lamarck proposed inheritance of acquired characters, Mendel discovered laws of heredity, De Vries proposed mutation theory. Darwin's theory emphasizes differential reproductive success of variants.
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