The technique used to determine the nucleotide sequence of DNA is: MCQ with Answer and Explanation

The technique used to determine the nucleotide sequence of DNA is:
A. DNA sequencing
B. PCR
C. Southern blotting
D. Gel electrophoresis
Answer: Option A
Solution (By JKSSB Mock Tests)
DNA sequencing determines the precise order of nucleotides in a DNA fragment. Sanger's dideoxy chain-termination method and next-generation sequencing are common. PCR amplifies DNA, gel electrophoresis separates by size, Southern blotting detects specific sequences. Sequencing is fundamental in genomics.

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Practice More Biology Questions

Question #1
In the human body, the 'Medulla oblongata' is a vital part of the brainstem that controls involuntary functions such as:
A. Vision and hearing
B. Balance and coordination
C. Heartbeat, breathing, and blood pressure
D. Memory and learning

Correct Answer: Option C


Explanation:
The medulla oblongata is the lowest part of the brainstem, connecting the pons to the spinal cord. It contains vital autonomic centers that control essential, involuntary life-sustaining functions. These include the cardiovascular center (regulating heart rate and blood vessel diameter), the respiratory center (regulating the rate and depth of breathing), and reflex centers for vomiting, coughing, sneezing, and swallowing. Damage to the medulla is often fatal due to the cessation of these vital functions.

This question belongs to: Science Biology
Question #2
Which branch of biology deals with the study of heredity and variation?
A. Ecology
B. Paleontology
C. Genetics
D. Embryology

Correct Answer: Option C


Explanation:
Genetics is the science of heredity and variation, explaining how traits are passed from parents to offspring and why individuals differ. Ecology studies organisms and environment, embryology deals with development, paleontology with fossils. Gregor Mendel is the father of genetics. The term 'genetics' was coined by William Bateson.

This question belongs to: Science Biology
Question #3
Consider statements about human anatomy: 1. The brain is the control center of the nervous system. 2. The heart is the pump of the circulatory system. 3. The lungs are the organs of the respiratory system. Which are correct?
A. 1 and 2 only
B. 1, 2 and 3
C. 1 and 3 only
D. 2 and 3 only

Correct Answer: Option B


Explanation:
The brain is the central and most complex organ of the nervous system, processing sensory information, controlling motor functions, and regulating higher cognitive processes. The heart is a muscular organ that acts as the central pump of the circulatory system, propelling blood through the network of blood vessels to deliver oxygen and nutrients. The lungs are a pair of spongy, air-filled organs that are the primary components of the respiratory system, facilitating the vital exchange of oxygen and carbon dioxide. All statements are correct.

This question belongs to: Science Biology