A plasmid used as a cloning vector must have: MCQ with Answer and Explanation

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A plasmid used as a cloning vector must have:

A. A large size to hold more DNA

B. An origin of replication and a selectable marker

C. A single restriction site only

D. No antibiotic resistance genes

Answer: Option B

Solution (By JKSSB Mock Tests)

A plasmid used as a cloning vector in genetic engineering must have certain essential features: an Origin of Replication (ori) to ensure its replication within the host cell, a Selectable Marker (like an antibiotic resistance gene) to identify and eliminate non-transformants, and at least one unique Restriction Site (cloning site) for the insertion of the foreign DNA.

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Question #1

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Consider the following statements about the human heart:
1. The pericardium is a protective sac around the heart.
2. The endocardium lines the heart chambers.
3. The myocardium is the outermost layer.
Which of the above is/are correct?

A. 2 and 3 only

B. 1, 2, and 3

C. 1 and 2 only

D. 1 and 3 only

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Correct Answer: Option C

Explanation:
Pericardium is outer sac, endocardium inner lining. Myocardium is the middle muscular layer; the outermost is epicardium (visceral pericardium). So 3 is incorrect.

This question belongs to: Science Biology

Question #2

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The glomerulus is a cluster of:

A. Muscle fibers

B. Neurons

C. Capillaries

D. Alveolar sacs

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Correct Answer: Option C

Explanation:
The glomerulus is a tuft of fenestrated capillaries enclosed by the Bowman's capsule in the renal corpuscle. Blood is filtered under high pressure through the capillary walls into the Bowman's space. The filtrate then passes into the nephron tubules. Glomerular filtration is the first step in urine formation.

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Question #3

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In the light reaction of photosynthesis, which photosystem is involved in the photolysis of water?

A. Neither PSI nor PSII

B. Photosystem I (PSI)

C. Photosystem II (PSII)

D. Both PSI and PSII

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Correct Answer: Option C

Explanation:
Photosystem II (PSII), also known as P680, is the first photosystem in the non-cyclic electron transport pathway (Z-scheme). It is responsible for the photolysis of water, a process where water molecules are split into oxygen, protons (H+), and electrons. This oxygen is released as a byproduct of photosynthesis.

This question belongs to: Science Biology

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