A plasmid used as a cloning vector must have: MCQ with Answer and Explanation

A plasmid used as a cloning vector must have:
A. An origin of replication and a selectable marker
B. A large size to hold more DNA
C. A single restriction site only
D. No antibiotic resistance genes
Answer: Option A
Solution (By JKSSB Mock Tests)
A plasmid used as a cloning vector in genetic engineering must have certain essential features: an Origin of Replication (ori) to ensure its replication within the host cell, a Selectable Marker (like an antibiotic resistance gene) to identify and eliminate non-transformants, and at least one unique Restriction Site (cloning site) for the insertion of the foreign DNA.

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Practice More Biology Questions

Question #1
The outermost layer of the skin is called the:
A. Dermis
B. Endodermis
C. Epidermis
D. Hypodermis

Correct Answer: Option C


Explanation:
The epidermis is the avascular, outermost layer of skin, composed mainly of keratinized stratified squamous epithelium. It provides a waterproof barrier and creates skin tone via melanocytes. The dermis is below, containing vessels and nerves, hypodermis is subcutaneous fat, endodermis is a root layer in plants.

This question belongs to: Science Biology
Question #2
Consider statements about biotechnology: 1. Agrobacterium tumefaciens is a natural plant genetic engineer. 2. Bt toxin is active in the acidic gut of insects. 3. RNAi is used to silence specific genes. Which are correct?
A. 1 and 3 only
B. 1 and 2 only
C. 1, 2 and 3
D. 2 and 3 only

Correct Answer: Option A


Explanation:
Agrobacterium tumefaciens is a soil bacterium that naturally transfers a segment of its Ti plasmid into plant cells, making it a natural genetic engineer used to create transgenic plants. RNA interference (RNAi) is a cellular defense mechanism used in biotechnology to silence specific mRNA molecules, preventing gene expression. Bt toxin is inactive in the insect gut until it encounters the alkaline pH, which solubilizes and activates it. Thus, statements 1 and 3 are correct.

This question belongs to: Science Biology
Question #3
The pitcher plant (Nepenthes) has modified leaves to trap insects primarily to obtain:
A. Water
B. Carbohydrates
C. Nitrogen
D. Oxygen

Correct Answer: Option C


Explanation:
The pitcher plant is an insectivorous (carnivorous) plant. Its leaves are modified into pitcher-shaped structures to trap and digest insects. This adaptation is primarily to supplement the plant's nitrogen intake, as it typically grows in nitrogen-deficient soils (like bogs). The digested insects provide essential nitrogenous compounds.

This question belongs to: Science Biology