A plasmid used as a cloning vector must have: MCQ with Answer and Explanation

A plasmid used as a cloning vector must have:
A. An origin of replication and a selectable marker
B. A single restriction site only
C. A large size to hold more DNA
D. No antibiotic resistance genes
Answer: Option A
Solution (By JKSSB Mock Tests)
A plasmid used as a cloning vector in genetic engineering must have certain essential features: an Origin of Replication (ori) to ensure its replication within the host cell, a Selectable Marker (like an antibiotic resistance gene) to identify and eliminate non-transformants, and at least one unique Restriction Site (cloning site) for the insertion of the foreign DNA.

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Practice More Biology Questions

Question #1
The process by which traits are transferred from parents to offspring is:
A. Evolution
B. Mutation
C. Heredity
D. Variation

Correct Answer: Option C


Explanation:
Heredity (inheritance) is the transmission of genetic information and traits from parents to offspring through genes. Variation refers to differences among individuals, evolution is the change in heritable traits over generations, and mutation is a change in DNA sequence. Mendel's laws describe the patterns of heredity. Genes are the units of heredity.

This question belongs to: Science Biology
Question #2
The process by which plants lose water in the form of liquid from hydathodes is:
A. Evaporation
B. Transpiration
C. Bleeding
D. Guttation

Correct Answer: Option D


Explanation:
Guttation is the exudation of water droplets containing dissolved minerals from hydathodes at leaf margins, driven by root pressure when transpiration is low. Transpiration is vapor loss, bleeding is sap from cut tissue.

This question belongs to: Science Biology
Question #3
Assertion (A): The appendix is a vestigial organ.
Reason (R): It has no known function in the human body.
A. Both A and R are true, and R is the correct explanation.
B. A is true, but R is false.
C. A is false, but R is true.
D. Both A and R are true, but R is not the correct explanation.

Correct Answer: Option B


Explanation:
The appendix is considered vestigial (reduced and non-essential), but recent studies suggest it may have a minor immune function or serve as a reservoir for gut bacteria. So R is not entirely true (it may have a function). Thus, A is true but R is false (no function is an oversimplification).

This question belongs to: Science Biology