Consider statements about biotechnology: 1. PCR can amplify a specific DNA sequence. 2. Gel electrophoresis separates DNA fragments by size. 3. Restriction enzymes join DNA fragments. Which are correct? MCQ with Answer and Explanation

Consider statements about biotechnology: 1. PCR can amplify a specific DNA sequence. 2. Gel electrophoresis separates DNA fragments by size. 3. Restriction enzymes join DNA fragments. Which are correct?
A. 1 and 3 only
B. 1 and 2 only
C. 2 and 3 only
D. 1, 2 and 3
Answer: Option B
Solution (By JKSSB Mock Tests)
Polymerase Chain Reaction (PCR) is a powerful technique used to exponentially amplify a specific segment of DNA using primers and a thermostable DNA polymerase. Gel electrophoresis is a method used to separate DNA, RNA, or protein molecules based on their size and charge by applying an electric field through a gel matrix. Restriction enzymes are molecular scissors that cut DNA at specific sequences; DNA ligase is the enzyme that joins DNA fragments together. Thus, statements 1 and 2 are correct.

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Question #1
The hormone that opposes the action of insulin and increases blood glucose levels is:
A. Growth hormone
B. Thyroxine
C. Cortisol
D. Glucagon

Correct Answer: Option D


Explanation:
Glucagon, from the pancreatic alpha cells, raises blood glucose by stimulating glycogenolysis and gluconeogenesis in the liver. Cortisol and growth hormone also raise blood glucose but glucagon is the primary direct antagonist of insulin. Thyroxine increases metabolic rate. The balance between insulin (hypoglycemic) and glucagon (hyperglycemic) maintains glucose homeostasis.

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Question #2
Rhizobium is used as a biofertilizer primarily for:
A. Decomposing organic matter
B. Producing auxins
C. Solubilizing phosphates
D. Fixing atmospheric nitrogen in legume root nodules

Correct Answer: Option D


Explanation:
Rhizobium is a symbiotic nitrogen-fixing bacterium that forms root nodules in the roots of leguminous plants (like peas, beans, clover). Inside these nodules, the bacterium converts atmospheric nitrogen (N2) into ammonia (NH3), which the plant can use. In return, the plant provides the bacteria with carbohydrates and a protected environment. It significantly reduces the need for chemical nitrogen fertilizers.

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Question #3
Consider statements about human diseases: 1. Influenza is caused by a virus. 2. Leprosy is caused by a fungus. 3. Malaria is transmitted by Anopheles mosquito. Which are correct?
A. 1 and 2 only
B. 1, 2 and 3
C. 1 and 3 only
D. 2 and 3 only

Correct Answer: Option C


Explanation:
Influenza (flu) is a respiratory illness caused by the influenza virus. Malaria is a parasitic disease caused by Plasmodium and transmitted exclusively by the bite of an infected female Anopheles mosquito. Leprosy (Hansen's disease) is a chronic infectious disease caused by the bacterium Mycobacterium leprae, not a fungus. Thus, statements 1 and 3 are correct.

This question belongs to: Science Biology