Consider statements about biotechnology: 1. Restriction enzymes cut DNA at specific sites. 2. PCR is used to amplify DNA. 3. Gel electrophoresis separates proteins by size. Which are correct? MCQ with Answer and Explanation
Consider statements about biotechnology: 1. Restriction enzymes cut DNA at specific sites. 2. PCR is used to amplify DNA. 3. Gel electrophoresis separates proteins by size. Which are correct?
A. 1 and 3 only
B. 2 and 3 only
C. 1 and 2 only
D. 1, 2 and 3
Answer: Option C
Solution (By JKSSB Mock Tests)
Restriction endonucleases act as molecular scissors, cleaving DNA at specific palindromic recognition sequences. Polymerase Chain Reaction (PCR) is a technique used to amplify specific DNA segments exponentially. Gel electrophoresis is primarily used to separate DNA or RNA fragments based on size, though it can separate proteins (SDS-PAGE), the statement implies it's exclusively or primarily for proteins in general biotech context, but 1 and 2 are definitively core facts. Let's refine: Gel electrophoresis separates charged molecules; for DNA it's by size. Statements 1 and 2 are universally correct core principles.
Explanation:
Methanogens are strict anaerobic Archaebacteria that produce methane (biogas) as a metabolic byproduct in hypoxic environments. They are commonly found in the guts of ruminant animals (like cows and buffaloes) and in anaerobic sludge digesters. Halophiles thrive in high salt, thermoacidophiles in hot acidic environments, and cyanobacteria are photosynthetic Eubacteria.
Explanation:
The ozone layer in the stratosphere absorbs most of the Sun's harmful UV radiation. CFCs, not CO₂, cause its depletion. Ozone at ground level is a pollutant. So B is correct.
Explanation:
B lymphocytes differentiate into plasma cells that produce antibodies (immunoglobulins) against specific antigens, providing humoral immunity. T cells mediate cellular immunity, macrophages and neutrophils are phagocytes.
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