Consider statements about five kingdom classification: 1. Monera includes all prokaryotes. 2. Protista includes unicellular eukaryotes. 3. Fungi are photosynthetic autotrophs. Which are correct? MCQ with Answer and Explanation

Explanation:
Ion exchange chromatography separates proteins based on their net surface charge; positively charged proteins bind to cation exchangers, negatively to anion exchangers. Gel filtration separates by size, affinity by specific binding, SDS-PAGE by molecular weight.

Explanation:
Messenger RNA (mRNA) is transcribed from DNA and carries the codon sequence to ribosomes, where it is translated into a polypeptide. tRNA brings amino acids, rRNA forms ribosomal structure, snRNA involved in splicing.

Explanation:
Innate (natural) immunity is the non-specific defense mechanism present from birth, including physical barriers like skin and chemical barriers like stomach acid. Acquired (adaptive) immunity is highly specific and develops after the body is exposed to a specific pathogen or antigen, involving the production of antibodies and memory cells. Vaccines provide artificial immunity (specifically, artificial active immunity), not natural immunity, which is acquired through actual infection or from mother to child. Thus, statements 1 and 2 are correct.