In recombinant DNA technology, restriction enzymes are used to: MCQ with Answer and Explanation

In recombinant DNA technology, restriction enzymes are used to:
A. Cut DNA at specific recognition sequences
B. Join DNA fragments together
C. Insert DNA into a host cell
D. Amplify DNA
Answer: Option A
Solution (By JKSSB Mock Tests)
Restriction enzymes (restriction endonucleases) are molecular 'scissors' that cut DNA at specific, highly specific recognition sequences (palindromic sequences). They are essential tools in genetic engineering for cutting both the source DNA (gene of interest) and the vector DNA at specific sites, creating compatible ends for ligation. DNA ligase is used to join the fragments.

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Practice More Biology Questions

Question #1
In genetics, the 'Poly-A tail' is a sequence of adenine nucleotides added to the 3' end of eukaryotic mRNA. Its primary functions include:
A. Binding to the DNA template
B. Protecting the mRNA from degradation by exonucleases and facilitating its export from the nucleus and translation
C. Splicing out introns
D. Initiating translation

Correct Answer: Option B


Explanation:
The poly-A tail is a long chain of adenine nucleotides (typically 100-250) added to the 3' end of a pre-mRNA molecule during post-transcriptional processing in the nucleus. Its primary functions are to protect the mRNA molecule from enzymatic degradation in the cytoplasm (by exonucleases), to facilitate the export of the mature mRNA from the nucleus to the cytoplasm, and to promote translation by helping to recruit ribosomes. It also plays a role in determining the lifespan of the mRNA in the cytoplasm.

This question belongs to: Science Biology
Question #2
The 'Hormone Growth Hormone' (GH), also known as somatotropin, is secreted by the anterior pituitary. Its primary effects include:
A. Decreasing fat breakdown
B. Lowering blood glucose levels
C. Stimulating growth, cell reproduction, and cell regeneration, particularly in bones and muscles
D. Inhibiting protein synthesis

Correct Answer: Option C


Explanation:
Growth Hormone (GH), or somatotropin, is a peptide hormone secreted by the somatotroph cells of the anterior pituitary gland. Its primary effects are to stimulate growth, cell reproduction, and cell regeneration, particularly in bones (stimulating the epiphyseal plates) and skeletal muscle. It also has significant metabolic effects: it increases protein synthesis (anabolic), promotes the breakdown of fats (lipolysis) to use as energy, and raises blood glucose levels (diabetogenic effect) by reducing glucose uptake by cells. Its effects are largely mediated by Insulin-like Growth Factors (IGFs), primarily produced by the liver.

This question belongs to: Science Biology
Question #3
The plant cell wall is mainly composed of:
A. Cellulose
B. Chitin
C. Peptidoglycan
D. Lignin

Correct Answer: Option A


Explanation:
The primary cell wall of plants consists of cellulose microfibrils embedded in a matrix of hemicellulose and pectin. Secondary walls may contain lignin.

This question belongs to: Science Biology