In recombinant DNA technology, restriction enzymes are used to: MCQ with Answer and Explanation

In recombinant DNA technology, restriction enzymes are used to:
A. Join DNA fragments together
B. Amplify DNA
C. Cut DNA at specific recognition sequences
D. Insert DNA into a host cell
Answer: Option C
Solution (By JKSSB Mock Tests)
Restriction enzymes (restriction endonucleases) are molecular 'scissors' that cut DNA at specific, highly specific recognition sequences (palindromic sequences). They are essential tools in genetic engineering for cutting both the source DNA (gene of interest) and the vector DNA at specific sites, creating compatible ends for ligation. DNA ligase is used to join the fragments.

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Practice More Biology Questions

Question #1
The 'corpus luteum' in the ovary secretes large amounts of progesterone primarily to:
A. Maintain the uterine endometrium for potential implantation of a fertilized egg
B. Trigger the LH surge for ovulation
C. Stimulate the growth of ovarian follicles
D. Initiate menstruation

Correct Answer: Option A


Explanation:
After ovulation, the ruptured Graafian follicle transforms into a temporary endocrine structure called the corpus luteum. Its primary function is to secrete large amounts of progesterone (and some estrogen). Progesterone is crucial for maintaining the thickened, highly vascularized uterine endometrium (secretory phase), making it receptive for the implantation of a blastocyst. If fertilization does not occur, the corpus luteum degenerates, progesterone levels drop, and menstruation begins.

This question belongs to: Science Biology
Question #2
Assertion (A): The menstrual cycle ceases during pregnancy.
Reason (R): High levels of progesterone and estrogen inhibit FSH and LH release.
A. Both A and R are true, but R is not the correct explanation.
B. A true, R false.
C. A false, R true.
D. Both A and R are true, and R is the correct explanation.

Correct Answer: Option D


Explanation:
During pregnancy, high progesterone and estrogen from the corpus luteum and placenta suppress gonadotropin secretion, preventing follicular development and menstruation. R correctly explains A.

This question belongs to: Science Biology
Question #3
Assertion (A): The color of urine is due to urobilin.
Reason (R): Urobilin is a breakdown product of hemoglobin.
A. Both A and R are true, and R is the correct explanation.
B. A true, R false.
C. Both A and R are true, but R is not the correct explanation.
D. A false, R true.

Correct Answer: Option A


Explanation:
Urobilin is derived from bilirubin, a heme breakdown product, and gives urine its yellow color. R is true and explains A.

This question belongs to: Science Biology