In the recombinant plasmid pBR322, the 'rop' gene codes for a protein that: MCQ with Answer and Explanation

In the recombinant plasmid pBR322, the 'rop' gene codes for a protein that:
A. Confers resistance to tetracycline
B. Produces proteins that restrict the growth of the host cell
C. Helps in the replication of the plasmid
D. Confers resistance to ampicillin
Answer: Option C
Solution (By JKSSB Mock Tests)
pBR322 is a widely used cloning vector. The 'rop' gene (Repressor Of Primer) codes for a small polypeptide that regulates and helps in the replication of the plasmid by inhibiting the synthesis of the RNA primer required for plasmid DNA replication. The 'ampR' and 'tetR' genes confer resistance to ampicillin and tetracycline, respectively, serving as selectable markers. Exam tip: 'rop' = replication control; 'R' = resistance.

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