The enzyme Taq polymerase, used in PCR, is obtained from: MCQ with Answer and Explanation

The enzyme Taq polymerase, used in PCR, is obtained from:
A. Salmonella typhimurium
B. Agrobacterium tumefaciens
C. Escherichia coli
D. Thermus aquaticus
Answer: Option D
Solution (By JKSSB Mock Tests)
Taq polymerase is a thermostable DNA polymerase enzyme isolated from the thermophilic bacterium Thermus aquaticus, found in hot springs. It is used in the Polymerase Chain Reaction (PCR) because it can withstand the high temperatures (around 94°C) required for the denaturation step of PCR without being destroyed, unlike normal DNA polymerases.

Discuss this Question (0)

No comments yet. Be the first to start the discussion!

Practice More Biology Questions

Question #1
The type of root system where many roots arise from the base of the stem, as in grasses, is called:
A. Taproot system
B. Fibrous root system
C. Adventitious root system
D. Prop root system

Correct Answer: Option B


Explanation:
Fibrous root systems consist of a dense network of thin, branching roots originating from the stem base, typical of monocots like grasses. Taproot system has a main central root (dicots). Adventitious roots arise from stems or leaves, prop roots are supportive. Fibrous roots are effective in preventing soil erosion.

This question belongs to: Science Biology
Question #2
The organelle that is the site of oxidative phosphorylation is:
A. Mitochondrion
B. Ribosome
C. Nucleus
D. Golgi apparatus

Correct Answer: Option A


Explanation:
Oxidative phosphorylation occurs on the inner mitochondrial membrane, where the electron transport chain creates a proton gradient, and ATP synthase uses this gradient to produce ATP. This is the major source of cellular energy. Nucleus stores DNA, Golgi processes proteins, ribosomes synthesize proteins.

This question belongs to: Science Biology
Question #3
In genetic engineering, the enzyme used to cut DNA at specific sequences is:
A. DNA polymerase
B. Helicase
C. Ligase
D. Restriction endonuclease

Correct Answer: Option D


Explanation:
Restriction endonucleases (restriction enzymes) recognize specific palindromic DNA sequences and cleave the phosphodiester bonds, producing sticky or blunt ends. They are essential tools for recombinant DNA technology. DNA polymerase synthesizes DNA, ligase joins fragments, helicase unwinds. Examples: EcoRI, HindIII. These enzymes are naturally present in bacteria as a defense against viruses.

This question belongs to: Science Biology