In genetic engineering, the enzyme used to cut DNA at specific sequences is: MCQ with Answer and Explanation

In genetic engineering, the enzyme used to cut DNA at specific sequences is:
A. Restriction endonuclease
B. Ligase
C. Helicase
D. DNA polymerase
Answer: Option A
Solution (By JKSSB Mock Tests)
Restriction endonucleases (restriction enzymes) recognize specific palindromic DNA sequences and cleave the phosphodiester bonds, producing sticky or blunt ends. They are essential tools for recombinant DNA technology. DNA polymerase synthesizes DNA, ligase joins fragments, helicase unwinds. Examples: EcoRI, HindIII. These enzymes are naturally present in bacteria as a defense against viruses.

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Practice More Biology Questions

Question #1
The organ that is the site of T-cell maturation is the:
A. Spleen
B. Thymus
C. Lymph node
D. Bone marrow

Correct Answer: Option B


Explanation:
The thymus gland is a primary lymphoid organ where T lymphocytes mature and become self-tolerant. Bone marrow is where B cells mature; spleen and lymph nodes are secondary.

This question belongs to: Science Biology
Question #2
Consider statements about human immunity: 1. Skin is a physical barrier. 2. Phagocytosis is a specific immune response. 3. Memory cells provide long-term immunity. Which are correct?
A. 1 and 2 only
B. 2 and 3 only
C. 1 and 3 only
D. 1, 2 and 3

Correct Answer: Option C


Explanation:
The skin and mucous membranes act as primary physical barriers in innate immunity, preventing pathogen entry. Phagocytosis by macrophages and neutrophils is a non-specific (innate) cellular defense mechanism, not a specific immune response. Memory B and T cells are produced during the primary specific immune response and provide rapid, long-lasting immunity upon re-exposure. Thus, statements 1 and 3 are correct.

This question belongs to: Science Biology
Question #3
The Chipko movement was primarily for the conservation of:
A. Forests
B. Wildlife
C. Water
D. Soil

Correct Answer: Option A


Explanation:
The Chipko movement (1970s, Uttarakhand) involved hugging trees to prevent them from being felled, promoting forest conservation.

This question belongs to: Science Biology