In genetic engineering, the enzyme used to cut DNA at specific sequences is: MCQ with Answer and Explanation

In genetic engineering, the enzyme used to cut DNA at specific sequences is:
A. Restriction endonuclease
B. Ligase
C. Helicase
D. DNA polymerase
Answer: Option A
Solution (By JKSSB Mock Tests)
Restriction endonucleases (restriction enzymes) recognize specific palindromic DNA sequences and cleave the phosphodiester bonds, producing sticky or blunt ends. They are essential tools for recombinant DNA technology. DNA polymerase synthesizes DNA, ligase joins fragments, helicase unwinds. Examples: EcoRI, HindIII. These enzymes are naturally present in bacteria as a defense against viruses.

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Practice More Biology Questions

Question #1
Consider statements about human anatomy: 1. The brain is part of the central nervous system. 2. The spinal cord is part of the peripheral nervous system. 3. Nerves are part of the peripheral nervous system. Which are correct?
A. 1, 2 and 3
B. 2 and 3 only
C. 1 and 2 only
D. 1 and 3 only

Correct Answer: Option D


Explanation:
The nervous system is divided into the central nervous system (CNS) and the peripheral nervous system (PNS). The CNS consists of the brain and the spinal cord, which process and coordinate information. The PNS consists of all the neural structures outside the CNS, including cranial nerves, spinal nerves, and ganglia, which connect the CNS to the rest of the body. Thus, statements 1 and 3 are correct.

This question belongs to: Science Biology
Question #2
Consider statements about animal husbandry: 1. Dairy farming involves milk production. 2. Poultry farming involves meat and egg production. 3. Apiculture involves silk production. Which are correct?
A. 1, 2 and 3
B. 1 and 3 only
C. 1 and 2 only
D. 2 and 3 only

Correct Answer: Option C


Explanation:
Dairy farming is the agricultural practice of breeding and raising livestock (like cows, buffaloes, goats) specifically for the long-term production of milk and milk products. Poultry farming involves the commercial rearing of domesticated birds (like chickens, ducks, turkeys) for the production of meat (broilers) and eggs (layers). Apiculture is the scientific rearing and management of honeybees (Apis species) for the production of honey, beeswax, and other hive products; silk production is sericulture. Thus, statements 1 and 2 are correct.

This question belongs to: Science Biology
Question #3
The disease caused by the bacterium Salmonella typhi is:
A. Cholera
B. Typhoid
C. Tuberculosis
D. Dysentery

Correct Answer: Option B


Explanation:
Typhoid fever is caused by Salmonella typhi, transmitted via contaminated food/water, causing high fever, abdominal pain, and rose spots. Widal test is used for diagnosis. Cholera is Vibrio cholerae, TB Mycobacterium, dysentery Shigella or Entamoeba.

This question belongs to: Science Biology