The method of separating proteins based on their size using an electric field is: MCQ with Answer and Explanation

The method of separating proteins based on their size using an electric field is:
A. SDS-PAGE
B. DNA sequencing
C. PCR
D. ELISA
Answer: Option A
Solution (By JKSSB Mock Tests)
SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) denatures proteins and coats them with negative charge, allowing separation by molecular weight in an electric field. PCR amplifies DNA, sequencing determines base order, ELISA detects antigens/antibodies. SDS-PAGE is a fundamental biochemistry technique.

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Practice More Biology Questions

Question #1
Who was the first scientist to observe bacteria using a single-lens microscope?
A. Antonie van Leeuwenhoek
B. Joseph Lister
C. Louis Pasteur
D. Robert Hooke

Correct Answer: Option A


Explanation:
Antonie van Leeuwenhoek (1670s) constructed high-quality single-lens microscopes and was the first to observe bacteria, protozoa, sperm cells, and red blood cells, describing them as 'animalcules'. Hooke observed cells in cork. Pasteur disproved spontaneous generation, Lister introduced antiseptic surgery. Leeuwenhoek's pioneering work laid the foundation for microbiology.

This question belongs to: Science Biology
Question #2
In the five-kingdom classification, the kingdom Protista includes:
A. Bacteria and cyanobacteria
B. Unicellular eukaryotes like amoeba and paramecium
C. Multicellular algae and bryophytes
D. Mushrooms and molds

Correct Answer: Option B


Explanation:
Protista includes unicellular eukaryotes such as protozoans (Amoeba, Paramecium), unicellular algae (Chlamydomonas, diatoms), and slime molds. They may be autotrophic or heterotrophic. Monera contains prokaryotes. Fungi include multicellular (except yeast) saprophytes. Plantae includes multicellular autotrophs. The protist kingdom is a 'catch-all' group for eukaryotes not fitting into fungi, plantae, or animalia.

This question belongs to: Science Biology
Question #3
The Fluid Mosaic Model of the cell membrane was proposed by which scientists?
A. Watson and Crick
B. Schleiden and Schwann
C. Singer and Nicolson
D. Robertson and Danielli

Correct Answer: Option C


Explanation:
The Fluid Mosaic Model of the plasma membrane was proposed by S. Jonathan Singer and Garth Nicolson in 1972. It describes the cell membrane as a dynamic, fluid structure composed of a phospholipid bilayer with globular proteins embedded or attached, moving laterally within the lipid matrix.

This question belongs to: Science Biology