The restriction enzyme EcoRI recognizes the palindromic DNA sequence 5'-GAATTC-3' and cuts the DNA between the: MCQ with Answer and Explanation

The restriction enzyme EcoRI recognizes the palindromic DNA sequence 5'-GAATTC-3' and cuts the DNA between the:
A. A and T
B. G and A
C. T and C
D. C and G
Answer: Option B
Solution (By JKSSB Mock Tests)
Restriction enzymes are molecular scissors that cut DNA at specific recognition sites. EcoRI, isolated from Escherichia coli strain RY13, recognizes the 6-base pair palindromic sequence 5'-GAATTC-3'. It cuts the phosphodiester bond between the G and the A on both strands, leaving single-stranded overhangs called 'sticky ends' (5'-AATT-3'). These sticky ends are crucial for joining DNA fragments from different sources using DNA ligase.

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Practice More Biology Questions

Question #1
Consider statements about microorganisms: 1. Bacteria can be beneficial or harmful. 2. Fungi are used in food production. 3. Viruses are living outside a host. Which are correct?
A. 1, 2 and 3
B. 2 and 3 only
C. 1 and 2 only
D. 1 and 3 only

Correct Answer: Option C


Explanation:
Bacteria exhibit diverse roles; many are beneficial (e.g., gut flora, nitrogen fixers, decomposers), while some are pathogenic and cause diseases. Fungi are extensively used in food production, such as yeast (Saccharomyces) in baking and brewing, and molds (Penicillium) in cheese making (e.g., Roquefort, Camembert). Viruses are considered non-living outside a host cell because they lack metabolic machinery and cannot replicate independently; they only exhibit living characteristics when inside a host. Thus, statements 1 and 2 are correct.

This question belongs to: Science Biology
Question #2
Which type of antibody is predominantly found in mucosal secretions, such as saliva, tears, and breast milk, providing localized immunity?
A. IgE
B. IgG
C. IgA
D. IgM

Correct Answer: Option C


Explanation:
Immunoglobulin A (IgA) is the primary antibody found in mucosal secretions, including saliva, tears, nasal secretions, breast milk (colostrum), and the respiratory and gastrointestinal tracts. It plays a crucial role in mucosal immunity by preventing the attachment of pathogens to epithelial cells, a process known as immune exclusion. IgG is the most abundant in blood, IgM is the first to be produced in an infection, and IgE is involved in allergies.

This question belongs to: Science Biology
Question #3
The neurotransmitter that is deficient in Parkinson's disease is:
A. Dopamine
B. Norepinephrine
C. Serotonin
D. Acetylcholine

Correct Answer: Option A


Explanation:
Parkinson's disease is a neurodegenerative disorder characterized by the loss of dopamine-producing neurons in the substantia nigra region of the brain. This leads to motor symptoms like tremors, rigidity, and bradykinesia. L-DOPA (levodopa), a precursor of dopamine, is used in treatment. Acetylcholine is related to Alzheimer's, serotonin to depression.

This question belongs to: Science Biology