The technique of creating a gene library by inserting DNA fragments into plasmid vectors and introducing into bacteria is: MCQ with Answer and Explanation

The technique of creating a gene library by inserting DNA fragments into plasmid vectors and introducing into bacteria is:
A. PCR
B. Microarray
C. DNA sequencing
D. Cloning
Answer: Option D
Solution (By JKSSB Mock Tests)
Molecular cloning involves inserting a DNA fragment into a vector (e.g., plasmid), which is then taken up by host cells (e.g., E. coli). As the bacteria replicate, they produce many copies of the inserted DNA, creating a gene library. PCR amplifies without vectors, sequencing reads bases, microarrays analyze expression.

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Practice More Biology Questions

Question #1
Consider statements about human nutrition: 1. A balanced diet contains all essential nutrients. 2. Malnutrition can be caused by overeating. 3. Water is not a nutrient. Which are correct?
A. 1 and 2 only
B. 1, 2 and 3
C. 2 and 3 only
D. 1 and 3 only

Correct Answer: Option A


Explanation:
A balanced diet provides all the essential nutrients (carbohydrates, proteins, fats, vitamins, minerals, and water) in the correct proportions required for optimal health and growth. Malnutrition refers to deficiencies, excesses, or imbalances in a person's intake of energy and/or nutrients; therefore, overeating (leading to obesity) is a form of malnutrition. Water is an essential nutrient, crucial for hydration, transport, and chemical reactions, though it provides no calories. Thus, statements 1 and 2 are correct.

This question belongs to: Science Biology
Question #2
Which type of antibody is predominantly found in mucosal secretions, such as saliva, tears, and breast milk, providing localized immunity?
A. IgM
B. IgG
C. IgA
D. IgE

Correct Answer: Option C


Explanation:
Immunoglobulin A (IgA) is the primary antibody found in mucosal secretions, including saliva, tears, nasal secretions, breast milk (colostrum), and the respiratory and gastrointestinal tracts. It plays a crucial role in mucosal immunity by preventing the attachment of pathogens to epithelial cells, a process known as immune exclusion. IgG is the most abundant in blood, IgM is the first to be produced in an infection, and IgE is involved in allergies.

This question belongs to: Science Biology
Question #3
The 'Urey-Miller experiment' synthesized which organic molecules?
A. Amino acids
B. Lipids
C. Proteins
D. DNA

Correct Answer: Option A


Explanation:
Stanley Miller and Harold Urey simulated early Earth conditions and obtained amino acids (like glycine and alanine) by applying electric discharge to a mixture of gases.

This question belongs to: Science Biology