The technique used to amplify small quantities of DNA in vitro is: MCQ with Answer and Explanation

The technique used to amplify small quantities of DNA in vitro is:
A. Polymerase chain reaction (PCR)
B. Gel electrophoresis
C. DNA sequencing
D. Southern blotting
Answer: Option A
Solution (By JKSSB Mock Tests)
PCR, invented by Kary Mullis, allows exponential amplification of a specific DNA segment using a thermostable DNA polymerase (Taq polymerase), primers, and thermal cycling. Gel electrophoresis separates DNA fragments, Southern blotting detects specific sequences, DNA sequencing determines the order of bases. PCR is widely used in diagnostics, forensics, and research.

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Practice More Biology Questions

Question #1
The infoldings of the inner mitochondrial membrane are called:
A. Cisternae
B. Thylakoids
C. Cristae
D. Grana

Correct Answer: Option C


Explanation:
Cristae are the numerous infoldings of the inner mitochondrial membrane that increase surface area for oxidative phosphorylation and ATP synthesis. They contain ATP synthase complexes. Thylakoids are membrane structures in chloroplasts; grana are stacks of thylakoids. Cisternae are flattened membrane sacs of ER and Golgi. Cristae give the inner membrane its characteristic folded appearance.

This question belongs to: Science Biology
Question #2
The process of cell death that is programmed and essential for normal development is:
A. Autolysis
B. Phagocytosis
C. Necrosis
D. Apoptosis

Correct Answer: Option D


Explanation:
Apoptosis is a genetically controlled, energy-dependent process of programmed cell death that removes unwanted, damaged, or infected cells without causing inflammation. It is vital for development (e.g., separation of digits in fingers) and tissue homeostasis. Necrosis is traumatic cell death causing inflammation. Autolysis is self-digestion after death, phagocytosis is engulfment.

This question belongs to: Science Biology
Question #3
The respiratory centre in the human brain is located in the:
A. Cerebellum
B. Medulla oblongata
C. Cerebrum
D. Hypothalamus

Correct Answer: Option B


Explanation:
The medulla oblongata contains the respiratory rhythmicity centre that controls the rate and depth of breathing. The pons also has pneumotaxic and apneustic centres influencing breathing. The cerebrum can exert voluntary control, cerebellum coordinates movement, and hypothalamus regulates homeostasis.

This question belongs to: Science Biology