The technique used to amplify small quantities of DNA in vitro is: MCQ with Answer and Explanation

The technique used to amplify small quantities of DNA in vitro is:
A. Gel electrophoresis
B. Polymerase chain reaction (PCR)
C. DNA sequencing
D. Southern blotting
Answer: Option B
Solution (By JKSSB Mock Tests)
PCR, invented by Kary Mullis, allows exponential amplification of a specific DNA segment using a thermostable DNA polymerase (Taq polymerase), primers, and thermal cycling. Gel electrophoresis separates DNA fragments, Southern blotting detects specific sequences, DNA sequencing determines the order of bases. PCR is widely used in diagnostics, forensics, and research.

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Practice More Biology Questions

Question #1
Identify the INCORRECT statement about the digestive system:
A. Pepsin is active in the acidic environment of the stomach.
B. Villi increase the surface area for absorption in the small intestine.
C. Trypsin is secreted by the pancreas.
D. Bile is produced in the gall bladder.

Correct Answer: Option D


Explanation:
Bile is produced by the liver and stored in the gall bladder. The gall bladder does not produce bile. All other statements are correct.

This question belongs to: Science Biology
Question #2
The female sex hormone produced by the corpus luteum is:
A. Estrogen
B. Prolactin
C. Progesterone
D. Testosterone

Correct Answer: Option C


Explanation:
The corpus luteum, formed after ovulation, secretes progesterone (and some estrogen) to maintain the uterine lining for pregnancy. If no fertilization, it degenerates, causing menstruation. Estrogen is also produced by growing follicles, testosterone by Leydig cells, prolactin by anterior pituitary.

This question belongs to: Science Biology
Question #3
In genetics, the 'Operon model' was proposed by:
A. Watson and Crick
B. McClintock
C. Beadle and Tatum
D. Jacob and Monod

Correct Answer: Option D


Explanation:
The Operon model, specifically the lac operon model, was proposed by French scientists François Jacob and Jacques Monod in 1961 to explain the regulation of gene expression in prokaryotes (bacteria). They proposed that genes are organized into functional units called operons, consisting of a promoter, an operator, and structural genes, controlled by a regulatory gene. They were awarded the Nobel Prize in Physiology or Medicine in 1965 for this discovery. Beadle and Tatum proposed the 'one gene-one enzyme' hypothesis.

This question belongs to: Science Biology