The technique used to detect specific proteins in a tissue section using labeled antibodies is: MCQ with Answer and Explanation

The technique used to detect specific proteins in a tissue section using labeled antibodies is:
A. Flow cytometry
B. ELISA
C. Western blotting
D. Immunohistochemistry
Answer: Option D
Solution (By JKSSB Mock Tests)
Immunohistochemistry (IHC) uses enzyme- or fluorophore-linked antibodies to visualize specific antigens in tissue sections. ELISA is for liquid samples, Western blot for membrane, flow cytometry for cells.

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Practice More Biology Questions

Question #1
In animal husbandry, the crossbreeding of superior males of one breed with superior females of another breed is called:
A. Crossbreeding
B. Grading up
C. Outcrossing
D. Inbreeding

Correct Answer: Option A


Explanation:
Crossbreeding involves mating individuals of different breeds to combine desirable traits and exploit hybrid vigor (heterosis). Inbreeding is within the same breed, outcrossing is within breed but unrelated, grading up is repeated crossing with a superior breed. Crossbreeding improves productivity in dairy and meat animals.

This question belongs to: Science Biology
Question #2
The process of separating proteins based on their net charge is:
A. Gel filtration chromatography
B. SDS-PAGE
C. Ion exchange chromatography
D. Affinity chromatography

Correct Answer: Option C


Explanation:
Ion exchange chromatography separates proteins based on their net surface charge; positively charged proteins bind to cation exchangers, negatively to anion exchangers. Gel filtration separates by size, affinity by specific binding, SDS-PAGE by molecular weight.

This question belongs to: Science Biology
Question #3
The vitamin that acts as a coenzyme in transamination reactions is:
A. Niacin
B. Thiamine
C. Pyridoxine (B₆)
D. Riboflavin

Correct Answer: Option C


Explanation:
Pyridoxal phosphate (PLP), the active form of vitamin B₆, is a coenzyme for transaminases (ALT, AST), decarboxylases, and other enzymes in amino acid metabolism. Thiamine in decarboxylation of keto acids, riboflavin in redox, niacin in NAD/NADP.

This question belongs to: Science Biology