The technique used to visualize and separate DNA fragments based on size is: MCQ with Answer and Explanation

The technique used to visualize and separate DNA fragments based on size is:
A. Gel electrophoresis
B. PCR
C. Microscopy
D. Centrifugation
Answer: Option A
Solution (By JKSSB Mock Tests)
Agarose gel electrophoresis separates DNA fragments by size as they move through the gel under an electric field; smaller fragments migrate faster. DNA is stained with ethidium bromide and visualized under UV light. PCR amplifies DNA, centrifugation separates by density, microscopy provides magnified images. Gel electrophoresis is essential in molecular biology and forensics.

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Practice More Biology Questions

Question #1
Assertion (A): Bile juice contains digestive enzymes.
Reason (R): Bile emulsifies fats to aid their digestion.
A. A is true, but R is false.
B. Both A and R are true, but R is not the correct explanation.
C. A is false, but R is true.
D. Both A and R are true, and R is the correct explanation.

Correct Answer: Option C


Explanation:
Bile does not contain digestive enzymes; it contains bile salts that emulsify fats, increasing surface area for lipase action. Thus, A is false, R is true.

This question belongs to: Science Biology
Question #2
Which of the following is NOT a part of the endomembrane system?
A. Golgi apparatus
B. Lysosomes
C. Mitochondria
D. Endoplasmic reticulum

Correct Answer: Option C


Explanation:
The endomembrane system includes ER, Golgi apparatus, lysosomes, vacuoles, and the nuclear envelope—organelles that coordinate membrane flow and protein processing. Mitochondria (and chloroplasts) are not part of this system because they are semi-autonomous, arising from endosymbiosis, and are not connected by vesicle trafficking. Peroxisomes are sometimes considered associated but not part of the ER-derived system.

This question belongs to: Science Biology
Question #3
Which of the following conditions is characterized by the presence of ketone bodies in urine, often associated with diabetes mellitus?
A. Ketonuria
B. Hematuria
C. Glycosuria
D. Proteinuria

Correct Answer: Option A


Explanation:
Ketonuria is the presence of ketone bodies (acetoacetate, beta-hydroxybutyrate, acetone) in urine, resulting from increased fat metabolism due to carbohydrate unavailability in uncontrolled diabetes mellitus. Glycosuria is glucose in urine, proteinuria is protein, hematuria is blood. Ketonuria indicates diabetic ketoacidosis, a medical emergency requiring immediate treatment.

This question belongs to: Science Biology