RNA interference (RNAi) is a technique used in genetic engineering to silence specific genes. In the development of nematode-resistant transgenic tobacco plants, the source of the silencing RNA was: MCQ with Answer and Explanation

RNA interference (RNAi) is a technique used in genetic engineering to silence specific genes. In the development of nematode-resistant transgenic tobacco plants, the source of the silencing RNA was:
A. A bacterium
B. The plant's own genome
C. The nematode itself (Agrobacterium-mediated delivery of nematode genes)
D. A virus
Answer: Option C
Solution (By JKSSB Mock Tests)
RNAi is a cellular defense mechanism in eukaryotes that silences specific mRNA. To create nematode-resistant tobacco, scientists used Agrobacterium as a vector to introduce nematode-specific genes into the host plant. The plant then produced both sense and anti-sense RNA for these genes. The double-stranded RNA (dsRNA) formed triggered the RNAi pathway, degrading the nematide's mRNA when it tried to express those essential genes inside the plant roots, killing the nematode.

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Question #1
The valve between the right atrium and right ventricle is the:
A. Pulmonary semilunar valve
B. Aortic semilunar valve
C. Tricuspid valve
D. Mitral valve

Correct Answer: Option C


Explanation:
The tricuspid valve has three cusps and prevents backflow of blood from the right ventricle to the right atrium during contraction. The mitral (bicuspid) valve is on the left side. Semilunar valves are at the ventricular outlets.

This question belongs to: Science Biology
Question #2
Consider statements about scientists: 1. Charles Darwin proposed the theory of evolution. 2. Gregor Mendel proposed the laws of inheritance. 3. Robert Hooke discovered the cell nucleus. Which are correct?
A. 1, 2 and 3
B. 1 and 3 only
C. 2 and 3 only
D. 1 and 2 only

Correct Answer: Option D


Explanation:
Charles Darwin, an English naturalist, proposed the theory of evolution by natural selection in his book 'On the Origin of Species'. Gregor Mendel, an Austrian monk, conducted experiments on pea plants and proposed the fundamental laws of inheritance (segregation and independent assortment). Robert Hooke discovered cells in cork tissue; the cell nucleus was discovered later by Robert Brown. Thus, statements 1 and 2 are correct.

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Question #3
Consider statements about human diseases: 1. Cholera is caused by a bacterium. 2. Polio is caused by a virus. 3. Ringworm is caused by a fungus. Which are correct?
A. 1 and 2 only
B. 1 and 3 only
C. 1, 2 and 3
D. 2 and 3 only

Correct Answer: Option C


Explanation:
Cholera is an acute diarrheal disease caused by the bacterium Vibrio cholerae, typically transmitted through contaminated water and food. Polio (poliomyelitis) is a highly infectious viral disease caused by the poliovirus, which can invade the nervous system and cause paralysis. Ringworm (dermatophytosis) is a common superficial fungal infection of the skin, hair, or nails caused by dermatophytes like Microsporum, Trichophyton, and Epidermophyton. All three statements correctly identify the causative agents.

This question belongs to: Science Biology